Identification of the Metastasis-associated, Galactoside-binding Lectin as a Chimeric Gene Product with Homology to an IgE-binding Protein1
نویسندگان
چکیده
We report the complete primary and secondary structures of a metas tasis-associated M, 34,000 galactoside-binding lectin. The polypeptide sequence (264 amino acids) was derived from the nucleotide sequence of three overlapping complementary DNA clones isolated from Xgtll and XgtlO phage libraries of UV-induced murine fibrosarcomas. Striking features of the polypeptide sequence are two distinct regions of /3-sheet and globular structures at the amino and carboxy terminals, respectively. Homology search suggests that the polypeptide is a chimeric gene product formed by fusion of the S'-end of an M, -14,000 galactoside-binding lectin with an internal domain of the collagen a gene. Enzymatic treatment with collagenase confirmed the presence of a collagen-like structure in the polypeptide. Unexpectedly, the entire sequence is >85% homologous to a rat low affinity IgE-binding protein.
منابع مشابه
IgE-binding Protein Lectin as a Chimeric Gene Product with Homology to an Identification of the Metastasis-associated, Galactoside-binding
We report the complete primary and secondary structures of a metas tasis-associated M, 34,000 galactoside-binding lectin. The polypeptide sequence (264 amino acids) was derived from the nucleotide sequence of three overlapping complementary DNA clones isolated from Xgtll and XgtlO phage libraries of UV-induced murine fibrosarcomas. Striking features of the polypeptide sequence are two distinct ...
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